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Mouse IFNAR1 ELISA Kit
Mouse IFNAR1 ELISA Kit
This ELISA kit is designed to detect Mouse IFNAR1. The assay plate has been pre-coated with mouse anti-Mouse IFNAR1 monoclonal antibody. When the sample containing IFNAR1 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse IFNAR1 Antibody is added to the wells and binds to IFNAR1 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse IFNAR1 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE323
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
50.22 pg/mL
Detection Range
140.62-9000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling, Immunology, Signal Transduction, Cancer
Background
Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulate Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant-negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Interferon alpha/beta receptor 1
Regulatory Status
For Research Use Only
