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Mouse Monocyte Differentiation Antigen CD14 (CD14) ELISA Kit

Mouse Monocyte Differentiation Antigen CD14 (CD14) ELISA Kit

This ELISA kit is designed to detect Mouse Monocyte Differentiation Antigen CD14 (Mouse CD14). The assay plate has been pre-coated with mouse anti-Mouse CD14 monoclonal antibody. When the sample containing CD14 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse CD14 Antibody is added to the wells and binds to CD14 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse CD14 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE282
Regular price $624.00 USD
Regular price $480.00 USD Sale price $624.00 USD
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Product Details

Species Reactivity Mouse
Sensitivity 26.94 pg/mL
Detection Range 78.13-5000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Immunology, Stem cells, Epigenetics and Nuclear Signaling
Background The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Monocyte differentiation antigen CD14
Regulatory Status For Research Use Only
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