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Mouse T-Cell Differentiation Antigen CD6 (CD6) ELISA Kit

Mouse T-Cell Differentiation Antigen CD6 (CD6) ELISA Kit

This ELISA kit is designed to detect Mouse T-Cell Differentiation Antigen CD6 (Mouse CD6). The assay plate has been pre-coated with mouse anti-Mouse CD6 monoclonal antibody. When the sample containing CD6 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse CD6 Antibody is added to the wells and binds to CD6 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse CD6 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE332
Regular price $624.00 USD
Regular price $480.00 USD Sale price $624.00 USD
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2 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 4.07 pg/mL
Detection Range 10.94-700 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Immunology, Stem cells
Background T-cell differentiation antigen CD6, also known as TP12 and CD6, is a single-pass type I membrane protein which contains threeSRCR domains. CD6 / TP12 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP12 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP12 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP12 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte T-cell differentiation antigen CD6
Regulatory Status For Research Use Only
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