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Mouse T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8a) ELISA Kit
Mouse T-Cell Surface Glycoprotein CD8 Alpha Chain (CD8a) ELISA Kit
This ELISA kit is designed to detect Mouse T-Cell Surface Glycoprotein CD8 Alpha Chain (Mouse CD8a). The assay plate has been pre-coated with mouse anti-Mouse CD8 alpha monoclonal antibody. When the sample containing CD8 alpha is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse CD8 alpha Antibody is added to the wells and binds to CD8 alpha in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse CD8 alpha present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE313
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
19.53 pg/mL
Detection Range
46.88-3000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Cancer, Immunology, Stem cells
Background
T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
T-cell surface glycoprotein CD8 alpha chain
Regulatory Status
For Research Use Only
