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Mouse Tissue Factor Pathway Inhibitor 2 (TFPI3) ELISA Kit

Mouse Tissue Factor Pathway Inhibitor 2 (TFPI3) ELISA Kit

This ELISA kit is designed to detect Mouse Tissue Factor Pathway Inhibitor 2 (Mouse TFPI3). The assay plate has been pre-coated with mouse anti-Mouse TFPI2 monoclonal antibody. When the sample containing TFPI2 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse TFPI2 Antibody is added to the wells and binds to TFPI2 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse TFPI2 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE336
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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2 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 2.84 pg/mL
Detection Range 7.81-500 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cardiovascular, Cell Biology
Background Tissue factor pathway inhibitor-2 (TFPI2), a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). It is a 32 kDa matrix-associated glycoprotein consisting of a short amino-terminal region, three tandem Kunitz-type domains and a positively charged carboxy-terminal tail. TFPI2 inhibits plasmin-dependent activation of several metalloproteinases. TFPI2 is highly abundant in the full-term placenta and widely expressed in various adult human tissues, such as the liver, skeletal muscle, heart, kidney, and pancreas. The expression of TFPI2 in tumors is inversely related to an increasing degree of malignancy, which may suggest a role for TFPI2 in the maintenance of tumor stability and inhibition of the growth of neoplasms. TFPI2 inhibits the tissue factor/factor VIIa (TF/VIIa) complex and a wide variety of serine proteinases including plasmin, plasma kallikrein, factor XIa, trypsin, and chymotrypsin. TFPI2 is involved in regulating pericellular proteases implicated in a variety of physiologic and pathologic processes including cancer cell invasion, vascular inflammation, and atherosclerosis. TFPI2 has also been shown to induce apoptosis and inhibit angiogenesis, which may contribute significantly to tumor growth inhibition.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Tissue factor pathway inhibitor 2
Regulatory Status For Research Use Only
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