• Retinol Binding Protein 4 (RBP4) ELISA
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Retinol Binding Protein 4 (RBP4) ELISA

Retinol Binding Protein 4 (RBP4) ELISA

The Retinol-Binding Protein 4 (RBP4) ELISA is an enzyme immunoassay for the quantitative determination of Retinol-Binding Protein 4 (RBP4) in human serum and plasma.

The microtiter plate is coated with the antibody specifically binding the Retinol-binding Protein 4. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of RBP4 in the specimen. The concentration of RBP4 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

Catalog No: BA1004
$560.00 USD
$560.00 USD $560.00 USD
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2 weeks

Product Details

Species Reactivity Human
Sensitivity 0.94 ng/mL
Detection Range 3.75 - 120 ng/mL
Sample Type Serum, Plasma, Urine
Sample Size 5 uL
Incubation(s) 2.5 hour(s)
Research Areas Nephrology, Metabolism
Background Retinol-binding Protein 4 (RBP4) is a member of the lipocalin family and the major transport protein of the hydrophobic molecule retinol, also known as vitamin A, in the circulation.1 Retinol-binding Protein 4 (RBP4) is elevated in serum and adipose tissue (AT) in obesity-induced insulin resistance and correlates inversely with insulin-stimulated glucose disposal. Serum RBP4 levels correlated inversely with glucose disposal and insulin-mediated suppression of lipolysis, FFA, and EGP.2 High levels of serum RBP4 are associated with chronic kidney disease (CKD) and urine BRP4 is the most sensitive biomarker of proximal renal tubule function.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
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