• sST2 ELISA
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sST2 ELISA

sST2 ELISA

The sST2 ELISA is an enzyme immunoassay for the quantitative determination of Soluble Suppression of Tumorigenicity 2 Protein (sST2) in human serum and plasma.

The microtiter plate is coated with the antibody specifically binding the sST2. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with HRP-labelled detection antibody. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue color. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of sST2 in the specimen. The concentration of sST2 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

Catalog No: BA1034
$560.00 USD
$560.00 USD $560.00 USD
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Packaging
2 weeks

Product Details

Species Reactivity Human
Sensitivity 0.02 ng/mL
Detection Range 0.188 - 6 ng/mL
Sample Type Serum, Plasma
Sample Size 10 uL
Incubation(s) 2 hour(s)
Research Areas Cardiovascular
Background sST2 (Soluble suppression of tumorigenicity 2) is a member of the Interleukin-1 receptor family. Like other ligands in the IL-1 family, the binding of IL-33 and ST2L on the inflammatory cell membrane activates subsequent intracellular signaling and mediates its pro-inflammatory action. It is raised in various cardiovascular diseases. sST2 significantly predicts disease severity and mortality in cardiovascular disease and is a good predictor of mortality in patients with stable coronary artery disease and chronic heart failure.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Soluble ST2
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