5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit
5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit
The 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 5-HIAA. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 5-HIAA. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HIAA in the samples is then determined by comparing the OD of the samples to the standard curve.
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