Ab1-42 (Amyloid Beta Peptide 1-42) ELISA Kit (Small Sample)
Ab1-42 (Amyloid Beta Peptide 1-42) ELISA Kit (Small Sample)
The Ab1-42 (Amyloid Beta Peptide 1-42) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.
Couldn't load pickup availability
Product Details
