The Bovine (CD8A) T-cell Surface Glycoprotein Cd8 Alpha Chain ELISA Kit measures T-Cell Surface Glycoprotein Cd8 Alpha Chain in Bovine samples. The plate has been pre-coated with Bovine CD8A antibody. CD8A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine CD8A Antibody is added and binds to CD8A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD8A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine CD8A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasImmunology, Cancer, CD & Adhesion molecule
BackgroundIntegral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I Peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. Source: UniProt Consortium (2025)
