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Bovine DEFB1 (Beta-defensin 1) ELISA Kit

Bovine DEFB1 (Beta-defensin 1) ELISA Kit

The Bovine (DEFB1) Beta-defensin 1 ELISA Kit measures Beta-defensin 1 in Bovine samples. The plate has been pre-coated with Bovine DEFB1 antibody. DEFB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine DEFB1 Antibody is added and binds to DEFB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DEFB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine DEFB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0111Bo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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Packaging
2.5 weeks
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Product Details

Species Reactivity Bovine
Sensitivity 0.54 ng/mL
Detection Range 1-400 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Has bactericidal activity. Active against E.coli ML35 but not against S.aureus 502A (PubMed:8454635). May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Beta-defensin 1
Regulatory Status For Research Use Only
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