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Bovine DNASE-1 (Deoxyribonuclease 1) ELISA Kit
Bovine DNASE-1 (Deoxyribonuclease 1) ELISA Kit
The Bovine (DNASE-1) Deoxyribonuclease 1 ELISA Kit measures Deoxyribonuclease 1 in Bovine samples. The plate has been pre-coated with Bovine DNASE1 antibody. DNASE1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine DNASE1 Antibody is added and binds to DNASE1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DNASE1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine DNASE1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0173Bo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Bovine
Sensitivity
3.91 ng/L
Detection Range
7-1500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling
Background
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed: 4976790, PubMed: 5166750, PubMed: 3352748, PubMed: 2395459). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed: 2395459). Binds specifically to G-actin and blocks actin polymerization (PubMed: 2395459). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Deoxyribonuclease 1
Regulatory Status
For Research Use Only
