Bovine E2 (Estradiol) ELISA Kit
Bovine E2 (Estradiol) ELISA Kit
This Estradiol ELISA kit is based on Competitive ELISA detection method and takes approximately 2 hours. The microplate provided in this kit has been precoated with E2. E2 in the sample or standard competes with a fixed amount of biotin-labeled E2 for sites on a pre-coated antibody specific to E2. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of E2 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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