Bovine FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit
Bovine FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit
The Bovine FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit measures FA/VB9 in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with FA/VB9. FA/VB9 in the sample or standard competes with a fixed amount of biotin-labeled FA/VB9 for sites on a pre-coated antibody specific to FA/VB9. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of FA/VB9 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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