The Bovine (GH) Growth Hormone ELISA Kit measures Growth hormone in Bovine samples. Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of GH in the sample. The concentration of GH in the sample is then determined by comparing the O.D. of the samples to the standard curve.
Research AreasSignal Transduction, Endocrinology, Developmental Biology
BackgroundGrowth hormone (GH) or somatotropin, also known as human growth hormones (hGH or HGH) in its human form, is a Peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. It is thus important in human development. GH also stimulates production of IGF-1 and increases the concentration of glucose and free fatty acids. It is a type of mitogen which is specific only to the receptors on certain types of cells. GH is a 191-amino acid, single-chain polypeptide that is synthesized, stored and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland. Source: UniProt Consortium (2025)
