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Bovine LPL (Lipoprotein Lipase) ELISA Kit

Bovine LPL (Lipoprotein Lipase) ELISA Kit

The Bovine (LPL) Lipoprotein Lipase ELISA Kit measures Lipoprotein Lipase in Bovine samples. The plate has been pre-coated with Bovine LPL antibody. LPL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine LPL Antibody is added and binds to LPL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LPL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine LPL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0411Bo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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Packaging
2.5 weeks
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Product Details

Species Reactivity Bovine
Sensitivity 0.095 ng/mL
Detection Range 0.2-70 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction
Background Key enzyme in triglyceride metabolism (PubMed: 9188470, PubMed: 16179346, PubMed: 10727238). Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed: 9188470, PubMed: 16179346, PubMed: 10727238). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (By similarity). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (By similarity). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed: 9188470). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Lipoprotein Lipase
Regulatory Status For Research Use Only
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