Skip to product information
1 of 1

Bovine OPN (Osteopontin) ELISA Kit

Bovine OPN (Osteopontin) ELISA Kit

The Bovine (OPN) Osteopontin ELISA Kit measures Osteopontin in Bovine samples. The plate has been pre-coated with Bovine OPN antibody. OPN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine OPN Antibody is added and binds to OPN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated OPN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine OPN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0402Bo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
Sale Sold out
Packaging
2.5 weeks
Download PDF

Product Details

Species Reactivity Bovine
Sensitivity 3.26 ng/mL
Detection Range 7-1500 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cardiovascular, Signal Transduction
Background Major non-collagenous bone protein that binds tightly to hydroxyapatite (Probable). Appears to form an integral part of the mineralized matrix (Probable). Probably important to cell-matrix interaction (Probable). Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Osteopontin
Regulatory Status For Research Use Only
View full details

Inquire about this product