Canine NT-proANP (N-Terminal Proatrial Natriuretic Peptide) ELISA Kit
Canine NT-proANP (N-Terminal Proatrial Natriuretic Peptide) ELISA Kit
This N-Terminal Proatrial Natriuretic Peptide ELISA kit is based on Sandwich ELISA detection method and takes approximately 4 hours. The microplate provided in this kit has been precoated with anti NT-proANP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with NT-proANP bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of NT-proANP in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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