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E. Coli Aconitate Hydratase B (acnB) ELISA Kit
E. Coli Aconitate Hydratase B (acnB) ELISA Kit
This ELISA kit is designed to detect E. Coli Aconitate Hydratase B (acnB). E. coli acnB. TThe assay plate has been pre-coated with mouse anti-E. coli acnB monoclonal antibody. When the sample containing acnB is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-E. coli acnB Antibody is added to the wells and binds to acnB in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of E. coli acnB present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE265
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
E. coli
Sensitivity
11.64 pg/mL
Detection Range
54.69-3500 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Signal Transduction
Background
Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Aconitate hydratase B
Regulatory Status
For Research Use Only
