This ELISA kit is designed to detect E. Coli Shiga Toxin Ii Subunit B. E. coli Shiga toxin II subunit B. TThe assay plate has been pre-coated with mouse anti-E. coli Shiga toxin II subunit B monoclonal antibody. When the sample containing Shiga toxin II subunit B is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-E. coli Shiga toxin II subunit B Antibody is added to the wells and binds to Shiga toxin II subunit B in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of E. coli Shiga toxin II subunit B present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
BackgroundE. Coli STX2B is a subunit of Stx2. Stx2, together with Stx1, formed a family of related toxins which are known as shiga toxins. Shiga toxins are mainly produced by the bacteria S. dysenteriae and the Shigatoxigenic group of Escherichia coli, which includes serotypes O157:H7, O104:H4, and other enterohemorrhagic E. coli (EHEC). A total of 3222 outbreak cases (including 39 deaths) have been reported in northern Germany in May through June 2011. The outbreak strain was typed as an enteroaggregative Shiga-toxin–producing E. coli O104:H4, producing extended-spectrum beta-lactamase. The toxin has two subunits—A and B. E. Coli STX2B is the B subunit. It is a pentamer that binds to specific glycolipids on the host cell, specifically globotriaosylceramide. Following this, the A subunit is internalised and cleaved into two parts. Stx2 has been found to be approximately 400 times more toxic (as quantified by LD50 in mice) than Stx-1. The Stx1 and Stx2 B subunits form a pentameric structure that binds to globotriaosylceramide receptors on eukaryotic cells and promotes endocytosis.
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
