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Human 15-Hydroxyprostaglandin Dehydrogenase [NAD(+)] (HPGD) ELISA Kit

Human 15-Hydroxyprostaglandin Dehydrogenase [NAD(+)] (HPGD) ELISA Kit

This ELISA kit is designed to detect Human 15-Hydroxyprostaglandin Dehydrogenase [NAD(+)] (HPGD). The assay plate has been pre-coated with mouse anti-Human 15-PGDH monoclonal antibody. When the sample containing 15-PGDH is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human 15-PGDH Antibody is added to the wells and binds to 15- PGDH in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human 15-PGDH present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE185
$754.00 USD
$580.00 USD $754.00 USD
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Product Details

Species Reactivity Human
Sensitivity 6.44 pg/mL
Detection Range 31.25-2000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Immunology, Signal Transduction, Neuroscience
Background 15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone, and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoarthropathy autosomal recessive (PHOAR), cranio-osteoarthropathy (COA), and isolated congenital nail clubbing.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte 15-hydroxyprostaglandin dehydrogenase [NAD
Regulatory Status For Research Use Only
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