The Human (A4GALT) Lactosylceramide 4-alpha-galactosyltransferase ELISA Kit measures Lactosylceramide 4-alpha-galactosyltransferase in samples. The plate has been pre-coated with Human A4GALT antibody. A4GALT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human A4GALT Antibody is added and binds to A4GALT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated A4GALT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human A4GALT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundCatalyzes the transfer of galactose from UDP-alpha-D-galactose to lactosylceramide/beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) to produce globotriaosylceramide/globoside Gb3Cer (d18:1(4E)) (PubMed:10748143).
Also able to transfer galactose to galactosylceramide/beta-D-Gal-(1<->1')-Cer (PubMed:10748143).
Globoside Gb3Cer is a glycosphingolipid of the globo serie, one of the major types of neutral root structures of glycosphingolipids, that constitute a significant portion of mammalian cell membranes (Probable). Globotriaosylceramide/globoside Gb3Cer in blood and tissue cell membranes is the antigen Pk of blood histogroup P (PubMed:10747952).
(Microbial infection) Globotriaosylceramide is one of the cellular ligands for bacterial verotoxins. Source: UniProt Consortium (2025)
