The Human (ACADSB) Short Branched Chain Specific Acyl-CoA Dehydrogenase ELISA Kit measures Short/branched chain specific acyl-CoA dehydrogenase in samples. The plate has been pre-coated with Human ACADSB antibody. ACADSB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ACADSB Antibody is added and binds to ACADSB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACADSB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ACADSB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundShort and branched chain specific acyl-CoA dehydrogenase that catalyzes the removal of one hydrogen from C-2 and C-3 of the fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed:7698750, PubMed:11013134, PubMed:21430231, PubMed:10832746).
Among the different mitochondrial acyl-CoA dehydrogenases, acts specifically on short and branched chain acyl-CoA derivatives such as (S)-2-methylbutyryl-CoA as well as short straight chain acyl-CoAs such as butyryl-CoA (PubMed:7698750, PubMed:11013134, PubMed:21430231, PubMed:10832746).
Plays an important role in the metabolism of L-isoleucine by catalyzing the dehydrogenation of 2-methylbutyryl-CoA, one of the steps of the L-isoleucine catabolic pathway (PubMed:11013134, PubMed:10832746).
Can also act on valproyl-CoA, a metabolite of valproic acid, an antiepileptic drug (PubMed:8660691). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteShort/branched chain specific acyl-CoA dehydrogenase
