1
/
of
1
Human ACTR3 (Actin-related Protein 3) ELISA Kit
Human ACTR3 (Actin-related Protein 3) ELISA Kit
The Human (ACTR3) Actin-related Protein 3 ELISA Kit measures Actin-Related Protein 3 in samples. The plate has been pre-coated with Human ACTR3 antibody. ACTR3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ACTR3 Antibody is added and binds to ACTR3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACTR3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ACTR3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4623Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
12.05 ng/L
Detection Range
20-4000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed: 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed: 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed: 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed: 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed: 17220302, PubMed: 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed: 29925947). Plays a role in ciliogenesis (PubMed: 20393563). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Actin-Related Protein 3
Regulatory Status
For Research Use Only
