The Human (ADAMTS5) A Disintegrin and Metalloproteinase with Thrombospondin Motifs 5 ELISA Kit measures A Disintegrin and Metalloproteinase with Thrombospondin Motifs 5 in samples. The plate has been pre-coated with Human ADAMTS5 antibody. ADAMTS5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ADAMTS5 Antibody is added and binds to ADAMTS5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADAMTS5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ADAMTS5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundMetalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN (PubMed: 16133547, PubMed: 18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration (By similarity). Participates in development of brown adipose tissue and browning of white adipose tissue (By similarity). Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteA Disintegrin and Metalloproteinase with Thrombospondin Motifs 5
