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Human AFP (Alpha-fetoprotein) ELISA Kit
Human AFP (Alpha-fetoprotein) ELISA Kit
The Human (AFP) Alpha-fetoprotein ELISA Kit measures Alpha-Fetoprotein in samples. The plate has been pre-coated with Human AFP antibody. AFP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AFP Antibody is added and binds to AFP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AFP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human AFP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1630Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.29 ng/mL
Detection Range
0.5-200 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cancer
Background
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Alpha-Fetoprotein
Regulatory Status
For Research Use Only
