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Human Beta-1,4-Galactosyltransferase 1 (B4GALT1) ELISA Kit
Human Beta-1,4-Galactosyltransferase 1 (B4GALT1) ELISA Kit
This ELISA kit is designed to detect Human Beta-1,4-Galactosyltransferase 1 (Human B4GALT1). The assay plate has been pre-coated with mouse anti-Human B4GALT1 monoclonal antibody. When the sample containing B4GALT1 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human B4GALT1 Antibody is added to the wells and binds to B4GALT1 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human B4GALT1 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE187
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
37.2 pg/mL
Detection Range
156.25-10000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Developmental Biology, Signal Transduction
Background
Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Beta-1,4-galactosyltransferase 1
Regulatory Status
For Research Use Only
