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Human CD28 (T-cell-specific Surface Glycoprotein Cd28) ELISA Kit

Human CD28 (T-cell-specific Surface Glycoprotein Cd28) ELISA Kit

The Human (CD28) T-cell-specific Surface Glycoprotein Cd28 ELISA Kit measures T-Cell-Specific Surface Glycoprotein Cd28 in samples. The plate has been pre-coated with Human CD28 antibody. CD28 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD28 Antibody is added and binds to CD28 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD28 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD28. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E4196hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.024 ng/mL
Detection Range 0.05-30 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Immunology, CD & Adhesion molecule
Background Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed: 8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed: 15067037). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte T-Cell-Specific Surface Glycoprotein Cd28
Regulatory Status For Research Use Only
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