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Human Cobalamin Binding Intrinsic Factor (CBLIF) ELISA Kit
Human Cobalamin Binding Intrinsic Factor (CBLIF) ELISA Kit
This ELISA kit is designed to detect Human Cobalamin Binding Intrinsic Factor (Human CBLIF). The assay plate has been pre-coated with mouse anti-Human Intrinsic Factor monoclonal antibody. When the sample containing Intrinsic Factor is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human Intrinsic Factor Antibody is added to the wells and binds to Intrinsic Factor in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human Intrinsic Factor present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE272
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
6.86 pg/mL
Detection Range
15.63-1000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Metabolism, Signal Transduction
Background
Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Cobalamin binding intrinsic factor
Regulatory Status
For Research Use Only
