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Human Cystatin-Sa (CST2) ELISA Kit

Human Cystatin-Sa (CST2) ELISA Kit

This ELISA kit is designed to detect Human Cystatin-Sa (Human CST2). The assay plate has been pre-coated with mouse anti-Human Cystatin SA monoclonal antibody. When the sample containing Cystatin SA is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human Cystatin SA Antibody is added to the wells and binds to Cystatin SA in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human Cystatin SA present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE204
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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Product Details

Species Reactivity Human
Sensitivity 0.87 pg/mL
Detection Range 4.69-300 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cell Biology
Background Cystatin-SA, also known as Cystatin-2, Cystatin-S5 and CST2, is a secreted protein which belongs to thecystatin family. Cystatin-2 / CST2 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in submandibular gland and parotid gland. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The CST1, CST2, CST4, and CST5 are expressed in differential, tissue-specific patterns. Expression of CST2 and CST5 is restricted to the submandibular and parotid glands, while CST1 and CST4 are expressed in these tissues and in the lacrimal gland.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Cystatin-SA
Regulatory Status For Research Use Only
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