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Human DGAT1 (Diacylglycerol-O-Acyltransferase Homolog 1) ELISA Kit
Human DGAT1 (Diacylglycerol-O-Acyltransferase Homolog 1) ELISA Kit
The Human (DGAT1) Diacylglycerol-O-Acyltransferase Homolog 1 ELISA Kit measures Diacylglycerol-O-Acyltransferase Homolog 1 in samples. The plate has been pre-coated with Human DGAT1 antibody. DGAT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DGAT1 Antibody is added and binds to DGAT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DGAT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human DGAT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2652Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
4.66 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed: 16214399, PubMed: 18768481, PubMed: 28420705, PubMed: 9756920, PubMed: 32433611, PubMed: 32433610). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed: 18768481). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed: 16214399). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (PubMed: 18768481). In contrast to DGAT2 it is not essential for survival (By similarity). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed: 16214399). Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester Synthases (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed: 28420705). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Diacylglycerol-O-Acyltransferase Homolog 1
Regulatory Status
For Research Use Only
