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Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit

Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit

This ELISA kit is designed to detect Human Epidermal Growth Factor Receptor (Human EGFR). The assay plate has been pre-coated with mouse anti-Human EGFR monoclonal antibody. When the sample containing EGFR is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human EGFR Antibody is added to the wells and binds to EGFR in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human EGFR present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE004
Regular price $624.00 USD
Regular price $480.00 USD Sale price $624.00 USD
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Product Details

Species Reactivity Human
Sensitivity 8.95 pg/mL
Detection Range 46.88-3000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cancer, Cell Biology, Microbiology, Neuroscience, Signal Transduction
Background As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Epidermal growth factor receptor
Regulatory Status For Research Use Only
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