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Human Ferritin Light Chain (FTL) ELISA Kit

Human Ferritin Light Chain (FTL) ELISA Kit

This ELISA kit is designed to detect Human Ferritin Light Chain (Human FTL). The assay plate has been pre-coated with mouse anti-Human Ferritin light chain monoclonal antibody. When the sample containing Ferritin light chain is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human Ferritin light chain Antibody is added to the wells and binds to Ferritin light chain in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human Ferritin light chain present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE255
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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Product Details

Species Reactivity Human
Sensitivity 9.81 pg/mL
Detection Range 46.88-3000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cardiovascular, Metabolism, Neuroscience, Signal Transduction
Background Ferritin, light polypeptide (FTL) is the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Storage of iron in the tissues occurs in the form of ferritin and hemosiderin. The latter originates from ferritin that has undergone intracellular digestion of its protein shell, leaving the iron core. Ferritin and hemosiderin are components of a continuum. Ferritin has been identified in all types of living organisms: animals, plants, molds, and bacteria. Whithin the protein shell of ferritin, iron is first oxidized to the ferric state for storage as ferric oxyhdroxide. Thus, ferritin removes excess iron from the cell sap where it could otherwise participate in peroxidation mechanisms.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Ferritin light chain
Regulatory Status For Research Use Only
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