1
/
of
1
Human HIF-3A (Hypoxia-inducible Factor 3 Alpha) ELISA Kit
Human HIF-3A (Hypoxia-inducible Factor 3 Alpha) ELISA Kit
The Human (HIF-3A) Hypoxia-inducible Factor 3 Alpha ELISA Kit measures Hypoxia-Inducible Factor 3 Alpha in samples. The plate has been pre-coated with Human HIF3A antibody. HIF3A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HIF3A Antibody is added and binds to HIF3A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HIF3A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HIF3A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4763Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
3.32 ng/L
Detection Range
6-1500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression (PubMed: 11573933, PubMed: 16126907, PubMed: 19694616, PubMed: 20416395, PubMed: 21069422). Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity). [Isoform 2]: Attenuates the ability of transcription factor HIF1A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. Also inhibits hypoxia-inducible ARNT-mediated gene expression. [Isoform 3]: Attenuates the ability of transcription factor HIF1A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. [Isoform 4]: Attenuates the ability of transcription factor HIF1A and EPAS1/HIF2A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation (PubMed: 16126907, PubMed: 17998805, PubMed: 19694616, PubMed: 20416395). May act as a tumor suppressor and inhibits malignant cell transformation (PubMed: 17998805). [Isoform 5]: Attenuates the ability of transcription factor HIF1A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Hypoxia-Inducible Factor 3 Alpha
Regulatory Status
For Research Use Only
