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Human IL-23 (Interleukin 23) ELISA Kit

Human IL-23 (Interleukin 23) ELISA Kit

The Human (IL-23) Interleukin 23 ELISA Kit measures Interleukin 23 in samples. The plate has been pre-coated with Human IL23 antibody. IL23 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL23 Antibody is added and binds to IL23 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL23 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL23. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0074Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 1.52 ng/L
Detection Range 3-900 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Immunology, Cytokines, Inflammation
Background Interleukin 23 (IL-23) is a member of the IL-12 cytokine family and composed of two subunits, IL-12 p40 and IL-23 p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called Th17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12RBeta1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-Beta and IL-6. The main biological effects of IL-23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages. This kit can be used to determine relative mass values for IL-23 p40. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Interleukin 23
Regulatory Status For Research Use Only
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