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Human IL-25 (Interleukin 25) ELISA Kit

Human IL-25 (Interleukin 25) ELISA Kit

The Human (IL-25) Interleukin 25 ELISA Kit measures Interleukin 25 in samples. The plate has been pre-coated with Human IL25 antibody. IL25 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL25 Antibody is added and binds to IL25 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL25 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL25. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0054Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 10.5 ng/L
Detection Range 20-6000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cytokines, Inflammation, Immunology
Background Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Interleukin 25
Regulatory Status For Research Use Only
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