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Human INOS-NOS2 (Nitric Oxide Synthase, Inducible) ELISA Kit
Human INOS-NOS2 (Nitric Oxide Synthase, Inducible) ELISA Kit
The Human (INOS-NOS2) Nitric Oxide Synthase, Inducible ELISA Kit measures Nitric Oxide Synthase, Inducible in samples. The plate has been pre-coated with Human NOS2 antibody. NOS2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NOS2 Antibody is added and binds to NOS2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NOS2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NOS2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4710Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.11 ng/mL
Detection Range
0.2-60 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience
Background
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed: 7531687, PubMed: 7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed: 25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed: 19688109). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Nitric Oxide Synthase, Inducible
Regulatory Status
For Research Use Only
