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Human Interleukin-6 (IL-6) ELISA Kit

Human Interleukin-6 (IL-6) ELISA Kit

This ELISA kit is designed to detect Human Interleukin-6 (Human IL-6). The assay plate has been pre-coated with mouse anti-Human IL-6 monoclonal antibody. When the sample containing IL-6 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human IL-6 Antibody is added to the wells and binds to IL-6 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human IL-6 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE397
Regular price $624.00 USD
Regular price $480.00 USD Sale price $624.00 USD
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2 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.58 pg/mL
Detection Range 3.13-200 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Neuroscience, Cancer, Immunology, Metabolism, Microbiology, Stem cells
Background Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Interleukin-6
Regulatory Status For Research Use Only
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