The Human (KCNJ10) Atp-sensitive Inward Rectifier Potassium Channel 10 ELISA Kit measures Atp-Sensitive Inward Rectifier Potassium Channel 10 in samples. The plate has been pre-coated with Human KCNJ10 antibody. KCNJ10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KCNJ10 Antibody is added and binds to KCNJ10 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KCNJ10 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human KCNJ10. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundMay be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium Channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the Channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules. Source: UniProt Consortium (2025)
