The Human (KCNJ11) Atp-sensitive Inward Rectifier Potassium Channel 11 ELISA Kit measures ATP-sensitive inward rectifier potassium Channel 11 in samples. The plate has been pre-coated with Human KCNJ11 antibody. KCNJ11 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KCNJ11 Antibody is added and binds to KCNJ11 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KCNJ11 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human KCNJ11. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundThis receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium (By similarity).
Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation. Source: UniProt Consortium (2025)
