The Human (KCNJ12) Atp-sensitive Inward Rectifier Potassium Channel 12 ELISA Kit measures ATP-sensitive inward rectifier potassium Channel 12 in samples. The plate has been pre-coated with Human KCNJ12 antibody. KCNJ12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KCNJ12 Antibody is added and binds to KCNJ12 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KCNJ12 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human KCNJ12. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundInward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Source: UniProt Consortium (2025)
