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Human LARP1 (La-related Protein 1) ELISA Kit
Human LARP1 (La-related Protein 1) ELISA Kit
The Human (LARP1) La-related Protein 1 ELISA Kit measures La-Related Protein 1 in samples. The plate has been pre-coated with Human LARP1 antibody. LARP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LARP1 Antibody is added and binds to LARP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LARP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human LARP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5556Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
7.96 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling
Background
RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed: 20430826, PubMed: 23711370, PubMed: 24532714, PubMed: 25940091, PubMed: 28650797, PubMed: 28673543, PubMed: 29244122). Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed: 23711370, PubMed: 25940091, PubMed: 28650797, PubMed: 29244122, PubMed: 26206669, PubMed: 28379136). The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed: 25940091, PubMed: 28650797, PubMed: 29244122, PubMed: 28379136). When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed: 25940091, PubMed: 28650797). Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed: 28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed: 20430826, PubMed: 24532714, PubMed: 25940091, PubMed: 28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed: 23711370, PubMed: 28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed: 20430826, PubMed: 24532714). Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed: 20430826, PubMed: 23711370, PubMed: 25940091, PubMed: 28673543). (Microbial infection) Positively regulates the replication of dengue virus (DENV). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
La-Related Protein 1
Regulatory Status
For Research Use Only
