The Human (LDLRAP1) Low Density Lipoprotein Receptor Adapter Protein 1 ELISA Kit measures Low density lipoprotein receptor adapter protein 1 in samples. The plate has been pre-coated with Human LDLRAP1 antibody. LDLRAP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LDLRAP1 Antibody is added and binds to LDLRAP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LDLRAP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human LDLRAP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundAdapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression (By similarity). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteLow density lipoprotein receptor adapter protein 1
