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Human LEPREL1 (Prolyl 3-hydroxylase 2) ELISA Kit
Human LEPREL1 (Prolyl 3-hydroxylase 2) ELISA Kit
The Human (LEPREL1) Prolyl 3-hydroxylase 2 ELISA Kit measures Prolyl 3-Hydroxylase 2 in samples. The plate has been pre-coated with Human LEPREL1 antibody. LEPREL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LEPREL1 Antibody is added and binds to LEPREL1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LEPREL1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human LEPREL1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5934Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
9.22 ng/L
Detection Range
20-6000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens (PubMed: 18487197). Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule (By similarity). Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1 (PubMed: 18487197). Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline (PubMed: 18487197). Has no activity on substrates that lack 4-hydroxyproline in the third position (PubMed: 18487197). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Prolyl 3-Hydroxylase 2
Regulatory Status
For Research Use Only
