The Human (LRP-4) Low-density Lipoprotein-receptor-related Protein 4 ELISA Kit measures Low-Density Lipoprotein-Receptor-Related Protein 4 in samples. The plate has been pre-coated with Human LRP4 antibody. LRP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LRP4 Antibody is added and binds to LRP4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LRP4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human LRP4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundMediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. May play an essential role in the process of digit differentiation (By similarity). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteLow-Density Lipoprotein-Receptor-Related Protein 4
