1
/
of
1
Human Lymphokine-Activated Killer T-Cell-Originated Protein Kinase (PBK) ELISA Kit
Human Lymphokine-Activated Killer T-Cell-Originated Protein Kinase (PBK) ELISA Kit
This ELISA kit is designed to detect Human Lymphokine-Activated Killer T-Cell-Originated Protein Kinase (Human PBK). The assay plate has been pre-coated with mouse anti-Human PBK monoclonal antibody. When the sample containing PBK is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human PBK Antibody is added to the wells and binds to PBK in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human PBK present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE126
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
21.19 pg/mL
Detection Range
62.5-4000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Signal Transduction
Background
PDZ binding kinase (PBK), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family, and has all the characteristic protein kinase subdomains and a C-terminal PDZ-binding T/SXV motif. PBK is expressed in the testis restrictedly expressed in outer cell layer of seminiferous tubules, as well as placenta. PBK may be enrolled in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. This mitotic kinase phosphorylates MAP kinase p38 and seems to be active in mitosis. When phosphorylated, PBK forms a protein-protein interaction with tumor suppressor p53 (TP53), leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. The expression level of PBK is thus upregulated in a variety of neoplasms including hematological malignancies.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Lymphokine-activated killer T-cell-originated protein kinase
Regulatory Status
For Research Use Only
