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Human LYPLA1 (Acyl-protein Thioesterase 1) ELISA Kit
Human LYPLA1 (Acyl-protein Thioesterase 1) ELISA Kit
The Human (LYPLA1) Acyl-protein Thioesterase 1 ELISA Kit measures Acyl-Protein Thioesterase 1 in samples. The plate has been pre-coated with Human LYPLA1 antibody. LYPLA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LYPLA1 Antibody is added and binds to LYPLA1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LYPLA1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human LYPLA1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4903Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
7.32 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Acts as a acyl-protein thioesterase (PubMed: 19439193, PubMed: 20418879). Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS (PubMed: 20418879). Has depalmitoylating activity toward KCNMA1 (PubMed: 22399288). Could also depalmitoylate ADRB2 (PubMed: 27481942). Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC) (PubMed: 19439193). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS) (By similarity). Has much higher thioesterase activity than lysophospholipase activity (PubMed: 19439193). Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA (PubMed: 21393252). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Acyl-Protein Thioesterase 1
Regulatory Status
For Research Use Only
