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Human Lysyl Oxidase Homolog 2 (LOXL2) ELISA Kit

Human Lysyl Oxidase Homolog 2 (LOXL2) ELISA Kit

This ELISA kit is designed to detect Human Lysyl Oxidase Homolog 2 (Human LOXL2). The assay plate has been pre-coated with mouse anti-Human LOXL2 monoclonal antibody. When the sample containing LOXL2 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human LOXL2 Antibody is added to the wells and binds to LOXL2 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human LOXL2 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE213
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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Product Details

Species Reactivity Human
Sensitivity 24.88 pg/mL
Detection Range 78.13-5000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cancer, Signal Transduction
Background Lysyl oxidase homolog 2, also known as Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14 and LOXL2, is a secreted protein that belongs to the lysyl oxidase family. LOXL2 contains four SRCR domains. The lysyl oxidase family is made up of five members: lysyl oxidase (LOX) and lysyl oxidase-like 1-4 ( LOXL1, LOXL2, LOXL3, LOXL4 ). All members share conserved C-terminal catalytic domains that provide for lysyl oxidase or lysyl oxidase-like enzyme activity; and more divergent propeptide regions. LOX family enzyme activities catalyze the final enzymatic conversion required for the formation of normal biosynthetic collagen and elastin cross-links. LOXL2 is expressed by pre-hypertrophic and hypertrophic chondrocytes in vivo, and that LOXL2 expression is regulated in vitro as a function of chondrocyte differentiation. LOXL2 promotes chondrocyte differentiation by mechanisms that are likely to include roles as both a regulator and an effector of chondrocyte differentiation. LOXL2 expression could also be explored as a molecular target in the prevention of breast cancer progression.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Lysyl oxidase homolog 2
Regulatory Status For Research Use Only
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