The Human (MANF) Mesencephalic Astrocyte-derived Neurotrophic Factor ELISA Kit measures Mesencephalic astrocyte-derived neurotrophic factor in samples. The plate has been pre-coated with Human MANF antibody. MANF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MANF Antibody is added and binds to MANF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MANF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MANF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundSelectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed:12794311).
Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity).
Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity).
Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed:18561914, PubMed:22637475, PubMed:29497057).
Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed:22637475).
Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475).
Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed:29497057).
Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed:29497057). Source: UniProt Consortium (2025)
