The Human (MAPRE1) Microtubule Associated Protein RP/EB Family Member 1 ELISA Kit measures Microtubule associated protein RP/EB family member 1 in samples. The plate has been pre-coated with Human MAPRE1 antibody. MAPRE1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MAPRE1 Antibody is added and binds to MAPRE1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MAPRE1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MAPRE1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPlus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570).
Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570).
May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes (PubMed:12388762).
Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570).
Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570).
Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242).
May play a role in cell migration (By similarity). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteMicrotubule associated protein RP/EB family member 1
